England bounced back from three consecutive defeats for the first time in 30 years as a much-changed side edged past Switzerland for a 1-0 friendly win in Leicester thanks to Marcus Rashford's victor.
And in England's 2-1 defeat to Spain in the Uefa Nations League on Saturday, Manchester United left-back Luke Shaw had to be taken off midway through the second half due to a head injury.
Both Gray and Chilwell had originally been in England's U21 squad which drew 0-0 with the Netherlands on Thursday and are set to play Latvia on Tuesday.
He played for 573 minutes at the World Cup this summer, picking up six goals and the Golden Boot along the way, and has started 61 matches in all competitions since the start of last season. But while there were some satisfactions - among them a first clean sheet in four games - England remain a work in progress.
He was one of only two players to keep their place as Gareth Southgate shuffled his pack at the King Power Stadium and a disjointed first-half showing followed.
Eric Dier, Ruben Loftus-Cheek and Fabian Delph will start in midfield, while Danny Welbeck joins Rashford up front.
England struggled to get Loftus-Cheek on the ball in unsafe positions in the first half and when they did, he was unable to do much with it, often showing an inability to turn when presented with the ball with his back to goal.
If England lose against Switzerland, it will be the nation's longest ever run of losses.
Kane was rested for England's friendly against Switzerland tonight, taking up a place on the bench.
The security provided by the presence of three central midfielders behind him enabled Shaqiri to roam wherever his little legs wished to carry him, and he popped up all over the pitch in the first half.