Along with "Notting Hill", she was known for a recurring role on the British series "The Vicar of Dibley" (1994-2007).
She also captured worldwide attention for her supporting role in the 1999 Hugh Grant and Julia Roberts comedy Notting Hill.
It read: "Emma created a wealth of characters and an vast body of work".
And broadcaster Emma Freud tweeted: "Our attractive friend Emma Chambers has died at the age of 53".
Emma Freud, the wife of The Vicar Of Dibley's creator, Richard Curtis, paid tribute to Chambers on Twitter. "Very sad news", he tweeted.
In 1998, Doncaster-born Chambers won the British Comedy Award for best actress for her performance in The Vicar Of Dibley. "And a tender, sweet, funny, unusual, loving human being".
Emma rose to fame portraying ditzy Alice in BBC One's famed sitcom, starring Dawn French, 60, as its lead.
Another posted: "That felt like a gut punch reading Emma Chambers has died".
A statement from her agent said she passed away from natural causes on Wednesday [February 21] and would be "greatly missed".
Chambers is known for playing the character of Honey in "Notting Hill". "I loved working with her, she was stunning", he said, according to the outlet.
For many, the closing credits, where Geraldine Granger told Alice a joke she didn't get, was the best part of the show.
While her sister Sarah Doukas discovered Kate Moss and ran the Storm modelling agency with brother Simon, Chambers always knew she wanted to be an actress. Very amusing, she made the daftness believable.