"Explicitly speaking, we have no intention to meet with the USA side during the stay in South Korea", read a statement attributed to Cho Yong Sam, director-general of the North American department of North Korea's foreign ministry, published in the state-controlled news agency KCNA on Thursday.
A thaw in relations between the two Koreas set in the beginning of the year.
Pyeongchang's opening ceremonies will be attended by North Korea's ceremonial leader, Kim Yong Nam, as well as Kim Yo Jong, the influential sister of leader Kim Jong Un, according to NPR's Elise Hu, who is reporting from Tokyo.
Vice President Mike Pence will lead an American delegation to the Games as part of a tour that includes stops in Alaska and Japan, and is bringing along Fred Warmbier, whose son Otto Warmbier died after being held in a North Korea prison.
The delegation is made up mostly of a 229-member cheer squad, along with 26 taekwondo performers, 21 journalists and four North Korean Olympics committee members.
Ms Kim was promoted to first vice director of the Central Committee of North Korea's ruling Workers' Party by her brother past year, which analysts said showed that her activities are more substantive and more important than previously thought.
North Korean leader Kim Jong-un's sister will visit South Korea for the Winter Olympics in PeyongChang, the South Korean ministry of national unification said.
"This raises worries that North Korea likely intends to use this Olympics as a propaganda tool rather than a possible opening to meaningful dialogue with South Korea".
South Korean President Moon Jae-in planned to meet the high-ranking delegation from the Democratic People's Republic of Korea (DPRK) on February 10, the presidential Blue House said Thursday.
US Vice President Mike Pence vowed any provocation by North Korea would result in a "response that is rapid, overwhelming and effective". The North's only airline, Air Koryo, is also blacklisted. (CBS News is owned by CBS, which also owns CNET.) Additionally, CBS says it's not sure whether the phones would work on networks inside North Korea after the games. She would be the first member of North Korea's ruling family to cross the border.