Mr Burnham appeared on stage with representatives of political parties, religions and both of the city's football clubs.
They sat at the steps of the stage in Manchester, mirroring their first performance, but this time in heels rather than animal onesies. The choir's cover of the song quickly went viral after they performed it in tribute to the Manchester bombing victims. But perhaps the most special moment - or at least the moment that's making Twitter emotional is when a Manchester police offer started dancing in a circle with a group of kids attending the concert.
To finish One Love Manchester, Grande gathered most of the night's performers onstage as she fought back tears to sing "One Last Time".
More than 14,000 tickets for the concert were set aside to be given free to fans who attended the original show. "I love you guys so much and I think that the kind of love and unity you're displaying is the kind of medicine that the world really needs right now".
All proceeds raised will be going towards a special fund set up for the families and the victims of the Manchester Arena attack which saw 22 people killed and 120 injured when a suicide bomber set of an explosive device after Ariana Grande performed at the venue on May 22. Backed by two singers and a guitarist, she delivered the song wearing all white, singing, "Throw your sticks and your stones, throw your bombs and your blows, but you're not gonna break my soul".
Previously, it was reported Ariana would take to the stage with the Black Eyed Peas to perform a special rendition of the band's single 'Where Is The Love?'.
Williams, who appeared to be wearing a sweatshirt from Justin Bieber's objective world tour, then treated the audience to his anthem Angels. "Just as I was about to leave the room, Adam looked at me and he said, 'Hey, Scooter, make sure you tell them, 'Don't go forward in anger, love spreads.' Adam, if you're watching, you're our hero tonight". "Manchester, I love you so so much, thank you so much. I wanna take this moment to honor the people that were lost, that were taken", he said. "When you look into the audience at my shows, you see a lovely, diverse, pure, happy crowd".
"It's taken quite a lot to come here today", said Alison Davies, from Warrington.
Ariana Grande organized the benefit show after 22 people were killed outside her concert last month.
"(God) loves you and he's here for you.
"We're broken-hearted for children who lost their parents and parents who lost their children from this senseless, senseless horror", he said.