Bolt has always thrived when competing in London and, in the stadium he won the last three of his six Olympic medals, he maintained his unbeaten record in the city with a real flourish, pulling away from the field with consummate ease.
Brushing aside any concerns about the hamstring injury which forced him to withdraw from his country's Olympic trials earlier this month, the Jamaican sprint king ran a solid time of 19.89sec in his first competitive 200m of this season in London on Friday.
"I felt it was a joke". "I'm just really happy and all the glory goes to God".
"I've had some hard races recently but hopefully I can iron out those mistakes for Rio", she told BBC Sport. "Good luck to the girls running in Rio, but I am just shocked now".
"I wanted to come out here and do what I know what I could have done [in Rio]", she said.
"I'm not fully in shape", Bolt told reporters. "I need more work but over time I will be fine".
The second and third places, meanwhile, went to Panama's Edward Alonso and Great Britain's Adam Gemili, who clocked 20.04 and 20.07 respectively.
The best performance of the night was Kendra Harrison who broke the 28-year-old women's 100m hurdles world record.
And as the Russian doping scandal threatens to overshadow the competition, the Jamaican is ready to deliver a good news story for track and field.
She collapsed into floods of tears at the finish at her achievement, clocking the revised scoreboard in the stadium.
Incredibly, Harrison will not be at next month's Rio Olympics, having finished sixth in the United States trials this month.
Usain Bolt and Kendra Harrison star at Muller Anniversary Games in London
Harrison's previous best was 12.24 seconds at the Eugene meet two months ago.
But worryingly for his competitors, this was Bolt's first 200m race for the season and to open with 19.89 seconds sends a strong message.
It smashed her season's best of 13.10secs and was her fastest time since the London Games, when she ran 12.54s. A hamstring injury, however, has hampered recent preparations meaning this was his first 200m outing of the year. "I needed races and that's why I'm really sad that I didn't get to compete at my trials because I needed those runs".
"That 12.40 (in the heats earlier on Friday) got my confidence back". "I had to give it all I had".
The best British performance of the night came from Laura Muir, who broke Kelly Holmes' national 1500m record with a hugely impressive run. I'm only 23. But it's right up there. It's not just any record.
Quick Take 3: Which Would You Rather Have - Olympic Gold Or The World Record? It's great to have.
It has been seven years since Bolt set the 200m world record of 19.19 seconds, and he is focused on lowering his time.
The old record was set by Bulgaria's Yordanka Donkova in 1988.
Over the course of her career, she was named an All-American 15 times.
Shaunae Miller of the Bahamas will also head to Rio in confident mood after posting a world-leading 49.55 to win the women's 400metres, setting up a mouth-watering one-lap showdown with Allyson Felix.